In their first outing without their talisman Bryn Snellgrove, Douglas went down 33-25 away at Dukinfield.
The game was always likely to be a potential banana skin and saw Douglas slip away from the leading pack at the top of South Lancashire/Cheshire Division Two.
They are now fourth, just nine points clear of fifth placed Vagabonds.
Football: Corinthians win big over newcomers
Douglas Royal make it six wins out of six
Rugby: Vagabonds Ladies extend lead atop league table
Douglas side win without conceding
Football: Wins for second division top three
Three points separate Castletown, Foxdale and Gymns
Atherton best FC Isle of Man at The Bowl
Ravens slump to back-to-back home defeats